2011-12-01

av MA Faridi · 2017 — Acoustophoresis, the ability to acoustically manipulate particles and cells inside a microfluidic channel, is a critical enabling technology for cell-sorting

Forskningsoutput: Tidskriftsbidrag › Artikel i vetenskaplig tidskrift Cushing K. et al., Reducing WBC background in cancer cell separation products by negative acoustic contrast particle immuno-acoustophoresis, Analytica Chimica Acta, 2018, 1000, 256e264 Page Manager: Thomas Laurell 2020-12-20 Acoustophoresis is a non-contact and label-free mode of manipulating particles and cell populations and allows for implementation of several separation modes [ 2 ]. The technology is currently finding increased applications in bioanalytical and clinical applications of cell handling and manipulation. Acoustophoresis is a microfluidic technology that uses ultrasound for the separation of cells based on their acoustophysical properties. In acoustophoresis, the movement of particles in liquids is controlled by a half‐wavelength ultrasound standing wave field generated across a microchannel (Fig. 1). Radiation forces in the acoustic standing wave field move the cell-bead complex faster to the center compared to non-target cells and can be separated in the center outlet of the channel (c).

Acoustophoresis cell separation

Biomedical Engineering, Dongguk University, Seoul, SOUTH KOREA ABSTRACT The basic principles of acoustic cell and particle manipulation in chip integrated acoustic standing … 2018-09-19 A novel method, free flow acoustophoresis (FFA), capable of continuous separation of mixed particle suspensions into multiple outlet fractions is presented. Acoustic forces are utilized to separate particles based on their size and density. The method is shown to be suitable for both biological and nonbiological suspended particles. Using this microchannel configuration, several publications have shown cancer cell lines (breast and prostate) being efficiently separated from WBCs from RBC-lysed blood using acoustophoresis with BASWs [3, 20].This label-free separation occurs because larger-sized cancer cells focus faster to the center pressure node than WBCs in the separation channel; thus, if flow rates are optimized the Acoustophoresis enables the label-free separation of functionally different subsets of cultured bone marrow stromal cells Franziska Olm , Hooi Ching Lim , Katharina Schallmoser , Dirk Strunk , Thomas Laurell & Stefan Scheding , 2020 Jun 15 , In : Cytometry. 2018-09-01 2017-12-07 Immunomagnetic cell separation is a technique whereby magnetic particles are used to isolate target cells from heterogeneous mixtures. To accomplish this, the magnetic particles are bound to specific cell surface proteins on the target cells via antibodies, enzymes, lectins, or streptavidin.

Binary separation of particle/vesicle/cell mixtures into two subpopulations is outlined based on the different polarities of the acoustic contrast factor. Furthermore, continuous flow separation of different particle/cell types is described where both Free Flow Acoustophoresis (FFA) and binary acoustophoresis …

79(14), 5117–5123 (2007) Google Scholar ACOUSTOPHORESIS -A SOUND APPROACH TO CHIP BASED CELL HANDLING – T. Laurell1,2 1 Dept. Measurement Science and Industrial Electrical Engineering Div. Nanobiotechnology, Lund University, Lund, SWEDEN 2 Dept.

Using this microchannel configuration, several publications have shown cancer cell lines (breast and prostate) being efficiently separated from WBCs from RBC-lysed blood using acoustophoresis with BASWs [3, 20].This label-free separation occurs because larger-sized cancer cells focus faster to the center pressure node than WBCs in the separation channel; thus, if flow rates are optimized the

acoustic energy density) during acoustophoretic separation. The ‘ Novel Cell Sorting and Separation Market: Focus on Acoustophoresis, Buoyancy, Dielectrophoresis, Magnetophoretics, Microfluidics, Optoelectronics, Traceless Affinity, and Other Technologies, 2019-2030 ’ report features an extensive study of the current landscape and future outlook of the growing market for novel cell sorting and separation technologies (beyond conventional methods). Hematopoietic progenitor cell assays revealed a preserved colony forming ability of nontarget cells post sorting.

177,178 As a suspension passes along a flow Lenshof, A., Laurell, T.: Continuous separation of cells and particles in microfluidic systems. Chem. Soc. Rev. 39(3), 1203–1217 (2010) Google Scholar Here, we investigated the performance of microfluidic acoustophoresis for the separation of CD8+ lymphocytes from PBPC, and present a method for affinity-bead-mediated acoustic separation of cells which otherwise cannot be discriminated acoustically. Acoustophoresis is a microfluidic technology that uses ultrasound for the separation of cells based on their acoustophysical properties. In acoustophoresis, the movement of particles in liquids is controlled by a half‐wavelength ultrasound standing wave field generated across a microchannel (Fig. 1 ). fractionation of red cells, platelets, and leukocytes.
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separation, buffer medium exchange, acoustic valving, affinity bead based extraction, acoustic trapping, cell interaction stud- ies, etc [4].

sorting, washing and separation of cells or micro-particles, and for mixing and For optimization of acoustophoresis (i.e. manipulation of particles into the It uses high-precision microfluidics to perform acoustophoretic cell separation, gene delivery by transduction or cell health. Gentle acoustophoresis improves cell yields and accelerates delivery to downstream process steps. Another emerging field of study where cell separation techniques are being blood cells using acoustic waves, referred to as acoustophoresis, Core Device i detta system är en akustofores chip som utvinner däggdjurscellstorlek (5-20 pm) partiklar från provet.
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Novel Cell Sorting and Separation Market: Focus on Acoustophoresis, Buoyancy-activated, Dielectrophoresis, Magnetophoretics, Microfluidics, Optoelectronics, Photoacoustics, Traceless Affinity and Other Technologies, 2019-2030 Novel Cell Sorting - Market research report and industry analysis - 12665139

The acoustic forces on the particle do not depend on properties such as pH, surface charge, or ionic strength. ACOUSTOPHORESIS SEPARATION OF BACTERIA FROM BLOOD CELLS FOR RAPID SEPSIS DIAGNOSTICS P. D. Ohlsson*, K. Petersson, P. Augustsson and T. Laurell Lund University, SWEDEN ABSTRACT We present a significantly improved acoustophoresis method to separate bacteria from red and white blood cells.